P-36: Evaluating The Effect of Lactobacillus Acidophilus and Bifidobacterium on Spermatogenesis Index of Testicular Tissue in Isoniazid-Induced Rats

Background: Isoniazid is an important drug in first line of treating the tuberculosis disease. This drug can lead to damages in different parts of the body such as liver and testis. Lactobacillus acidophilus and Bifidobacterium are the probiotics that have the important innate property that inhibit from passing of Isoniazid into the blood-testis-barrier. In point of spermatogenesis indexes which includes tubular differentiation index (TDI), spermatogenesis index (SI) and repopulation index (RI) were studied. Materials and Methods: In this study, 240 male adult Albino rats with 15 rats per each cage and four replicate for each group were used. The groups were as followed: 1. control group (Normal saline 10 ml/Kg orally); 2. Isoniazid group (were injected 50 mg/Kg IP followed for 3 weeks); 3. Isoniazid with probiotics (were injected Isoniazid 50 mg/Kg IP;administration: Lactobacillus acidophilus 2×[10]^9CFU/ml; Bifidobacterium 1×[10]^8 CFU/ml orally followed for 3 weeks); 4. Probiotics group (administration: Lactobacillus acidophilus and Bifidobacterium). The testis tissues was sampled after passing the mentioned time and section providing was stained by H andE. Results: The results showed that Lactobacillus acidophilus with Bifidobacterium significantly decreased (P<0.05) RI, SI and TDI indexes compared with control group. Consumption of Isoniazid without Lactobacillus acidophilus and Bifidobacterium significantly increased RI, SI, and TDI (P<0.05) compared with Isoniazid with these probiotics administered groups. Conclusion: Our results showed that RI, SI, and TDI were significantly changed after Isoniazid and Lactobacillus acidophilus consumption. These results are caused by reducing the serum testosterone concentration and the effect of Isoniazid and Lactobacillus acidophilus with Bifidobacterium in penetration of the blood-testisbarrier. It suggests the effect of Lactobacillus acidophilus and Bifidobacterium with Isoniazid on spermatogenesis index and infertility in human which needs more investigation.